In
its December 2017 issue, The Journal of
Food Composition and Analysis (JFCA) published an editorial in which it
announced that the journal “will no longer accept papers for review that employ
antioxidant and total phenolic assays.”1
The
JFCA’s new policy follows a trend started by other scientific journals that focus
on natural products research and that no longer accept or restrict acceptance
of papers dealing with antioxidant activity of extracts or isolates from plant,
fungal, or animal sources. The Journal of
Ethnopharmacology lists “in vitro antioxidant activity” as a rejection
criterion, stating that such activity is “present in all plants” and thus is less
meaningful without additional data.2 Planta Medica and Fitoterapia also reject manuscripts that report predictable
biological activities, such as the antioxidant activities of phenolic
compounds.3,4
In his editorial, JFCA Editor-in-Chief
James Harnly, PhD, a research leader at
the United States Department of Agriculture’s Food
Composition and Methods Development Laboratory, notes that there is
little evidence that antioxidant activities observed in vitro will have an
impact on health in animal or human studies.1 In addition, the
tests used to measure total antioxidant activity, such as the ORAC, DPPH, TEAC,
and FRAP† assays, are non-specific and prone to
interferences, and therefore do not provide
reliable results. As a consequence, the JFCA will immediately reject
papers in which the data primarily rely on these assays. For manuscripts that
use these assays to provide additional data (e.g., to explain a mechanism of
action), the JFCA will request that the authors re-submit the paper after
omission of the antioxidant data.1
Interest in antioxidant activities started
to grow in the late 1990s because of data that established the involvement of
reactive oxygen species (ROSs) in a number of major health issues (e.g.,
inflammation, cardiovascular disease, and cancer). The theory was that since
antioxidants were able to react with these ROSs in vitro, they might lead to the
development of agents capable of preventing some of the related health issues
in humans. According to PubMed — the extensive medical database maintained by
the US National Library of Medicine (a part of the US National Institutes of
Health) — the number of papers containing the term “antioxidant activity” in
the title or abstract has skyrocketed (Figure 1) over the past two decades.
Figure 1. Number of Papers with the Term 'Antioxidant Activity' in Their Title or Abstract Listed in PubMed, 1975-2015.
The usefulness of the data published on
antioxidant activities is rightfully a matter of debate in the scientific
community. Phenolic compounds, which play an important role in plant defense
mechanisms, occur widely in the plant kingdom.5,6 Plant defense
mechanisms are activated in response to plant pathogens, injury, or
environmental factors.5-7 The increase in the concentration of
ROSs is one of the initial defense reactions observed in plants. In order to
avoid damage caused by the increased concentrations of ROSs, plant tissues use
an array of antioxidant mechanisms, including enzymes and antioxidant secondary
metabolites.5,6 While these
antioxidant compounds play an important physiological role in plants, much of their
impact on human health has yet to be demonstrated.
Many dietary supplement and conventional
food manufacturers have products with antioxidant claims in their portfolio
(e.g., nutrition bars and antioxidant beverages), and the increased skepticism
about the value of results from in vitro antioxidant assays may have an impact
on these claims.8 It may become less
enticing for companies to make such claims if a majority of the scientific
community concludes that such data are meaningless, or — as written in the
editorial — that “‘antioxidant’
is a marketing term of questionable health and analytical value.”1
—Stefan Gafner, PhD
References
- Harnly
J. Antioxidant methods. J Food Comp Anal.
2017;64(Part 2):145-146.
- The
“Rules of 5.” Journal of
Ethnopharmacology website. Available at: www.elsevier.com/__data/promis_misc/jeprulesof5.pdf.
Accessed January 5, 2018.
- Guide
for authors. Fitoterapia website. Available
at: www.elsevier.com/journals/fitoterapia/0367-326x/guide-for-authors.
Accessed January 8, 2018.
- Guidelines
for authors: editorial policy. Planta
Medica website. Available at: www.thieme.com/media/ita/pubid-1019644585.pdf.
Accessed January 8, 2018.
- Kulbat
K. The role of phenolic compounds in plant resistance. Biotechnol Food Sci. 2016;80(2):97-108.
- Lattanzio
V, Lattanzio VMT, Cardinali A. Role of phenolics in the resistance mechanisms
of plants against fungal pathogens and insects. In: Imperato F, ed. Phytochemistry: Advances in Research.
Kerala, India: Research Signpost; 2006:23-67.
- Morales
LO, Tegelberg R, Brosché M, Keinänen M, Lindfors A, Aphalo PJ. Effects of solar
UV-A and UV-B radiation on gene expression and phenolic accumulation in Betula pendula leaves. Tree Physiol. 2010;30(7):923-934.
- ORAC
values might be passé, but antioxidant activity still demands research, experts
say. William Reed Business Media, Inc.; 2017. Available at:
www.nutraingredients-usa.com/Article/2017/08/15/ORAC-values-might-be-passe-but-antioxidant-activity-still-demands-research-experts-say.
Accessed January 2, 2018.
† ORAC: Oxygen Radical Absorbance Capacity;
DPPH: 2,2-Diphenyl-1-picrylhydrazyl; TEAC: Trolox Equivalent Antioxidant
Capacity; FRAP: Ferric Reducing Antioxidant Power
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