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- Chokeberry (Aronia melanocarpa, Rosaceae)
- Antioxidants
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Date:
03-31-2015 | HC# 091414-517
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Re: Chokeberry Juice Improves Antioxidant Status in Healthy Female Subjects
Kardum N, Konić-Ristić
A, Šavikin K, et al. Effects of polyphenol-rich chokeberry juice on
antioxidant/pro-oxidant status in healthy subjects. J Med Food. 2014;17(8):869-874.
Chokeberry (Aronia
melanocarpa, Rosaceae) fruits are a rich source of polyphenols, a class
of compounds associated with antioxidant activity. Although numerous studies
have evaluated the antioxidant effects of chokeberries in vitro, limited
information is available about the antioxidant effects of these berries in
human subjects. The aim of
this 12-week intervention study was to evaluate the antioxidant status of
healthy female subjects that consumed a polyphenol-rich organic chokeberry
juice over a 12-week period.
This study
took place at the University of Belgrade, Serbia. A total of 29 healthy female
subjects (aged 25-49 years) that were not taking medication or antioxidant
supplements were recruited for the study. Subjects consumed 100 ml of
polyphenol-rich organic chokeberry juice (Conimex Trade; Belgrade, Serbia.) per
day for 12 weeks. The total phenolic and total anthocyanin content in the
chokeberry beverage were 386 mg GAE/100 g of juice and 25 mg/100 g of juice,
respectively.
Dietary
habits were not altered for the study and were recorded by a food frequency
questionnaire. Standard biochemical tests and anthropometric parameters were determined
at baseline and after 12 weeks. Blood samples were obtained from subjects after
an overnight fast. Serum oxidative status was assessed at baseline and at the
end of the study by the following: thiobarbituric acid-reactive substances
(TBARS; a byproduct of lipid peroxidation), total antioxidative capacity (TAC),
total oxidative status (TOS), sulphydryl (-SH) groups, paraoxonase-1 (PON1) activity
toward paraoxon (POase), PON1
activity toward diazoxon (DZOase), and pro-oxidant-antioxidant balance (PAB).
The women in
the study had an average height of 168.7 ± 6.2 cm and weight of 65.1 ± 11.5 kg.
After completion of the study, no significant changes were found for
biochemical parameters, and all values remained in the acceptable reference
range. Although uric acid increased after the intervention, this effect was not
significant (P=0.08). In terms of antioxidant status, chokeberry juice
consumption significantly reduced the level of TBARS (P<0.001), PAB
(P<0.05), and TAC (P<0.05). Subjects also had non-significant reduced
levels of TOS (P=0.209), -SH groups (P=0.200), and POase (P=0.124). DZOase was significantly increased at the end of the study
(P<0.01). In addition, it was also found that TBARS correlated with age,
body mass index (BMI), waist circumference, and body fat, as well as systolic
and diastolic blood pressure.
This study
demonstrated that chokeberry consumption may improve antioxidant status based
on decreased oxidative damage (TBARS, TOS) and PAB, as well as increased DZOase
(protection against lipid oxidation). These results are consistent with 2 other
studies that found chokeberry consumption decreased TBARS.1,2
Interestingly, the authors note that this is the first study to evaluate the
PAB effects of chokeberry juice. In addition, the authors speculate that
because this study was conducted in healthy subjects, where there is a lower
concentration of reactive oxygen species, the anthocyanins (subclass of
polyphenolic compounds) from the berries may have exhibited a slight
pro-oxidant effect. To confirm the antioxidant benefits of chokeberry juice
found in this study, randomized controlled trials, evaluating similar oxidative
parameters, should be conducted in subjects with different health conditions
that are associated with oxidative stress.
—Laura M. Bystrom, PhD
References
1Pilaczynska-Szczesniak L, Skarpanska-Steinborn A,
Deskur E, Basta P, Horoszkiewicz-Hassan M. The influence of chokeberry juice
supplementation on the reduction of oxidative stress resulting from an
incremental rowing ergometer exercise. Int
J Sport Nutr Exerc Metab. 2005;15(1):48-58.
2Broncel
M, Kozirog M, Duchnowicz P, Koter-Michalak M, Sikora J, Chojnowska-Jezierska J.
Aronia melanocarpa extract reduces
blood pressure, serum endothelin, lipid, and oxidative stress marker levels in
patients with metabolic syndrome. Med Sci
Monit. 2010;16(1):CR28-CR34.
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