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- Black Chokeberry (Aronia melanocarpa, Rosaceae)
- Inflammation
- Exercise
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Date:
04-15-2015 | HC# 111411-518
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Re: Black Chokeberry Juice Improves Inflammation and Iron Metabolism Parameters after Intense Physical Exercise
Skarpańska-Stejnborn
A, Basta P, Sadowska J, Pilaczyńska-Szcześniak Ł. Effect of supplementation
with chokeberry juice on the inflammatory status and markers of iron metabolism
in rowers. J Int Soc Sports Nutr.
2014;11(1):48. doi: 10.1186/s12970-014-0048-5.
Exercise,
especially intense physical activity, has been shown to cause pro-oxidant
effects, inflammation, and a decrease in serum iron. Several studies have
indicated that black chokeberry (Aronia
melanocarpa, Rosaceae) fruit extracts have anti-inflammatory effects. Yet,
few studies have evaluated if flavonoid-rich foods, such as black chokeberry,
produce similar effects after exercise. The aim of this placebo-controlled
trial was to evaluate the effects of black chokeberry juice on pro-inflammatory cytokines, markers of
iron metabolism, and total antioxidant capacity (TAC) in rowers subjected to
exhaustive exercise.
This study
was conducted in Poland at an Olympic Games Training
Center (location undisclosed). A total of 19 male subjects from the Polish
rowing team (16 heavyweight and 3 lightweight rowers) participated in the study
during an 8-week training period. The intensity (based on lactic acid levels),
volume (minutes/day), and type of training were recorded on a daily basis. The
subjects' daily food and caloric intake were constant throughout the study
period and they did not consume any medication or supplements.
Participants were divided into 2 groups; one (n=10)
consumed 50 mL of black chokeberry juice (Europlant PhytoPharm Klęka S.A.; Nowe Miasto nad Wartą,
Poland) 3 times a day for 8 weeks, and a placebo group
(n=9) consumed a beverage identical in appearance and taste (6.6% betaine and 1%
citric acid solution) for the same amount of time. The phenolic (e.g.,
flavonoids) content of the chokeberry juice was also evaluated.
Two
assessments were given before and after supplementation, referred to as Trial I
and Trial II, respectively. The training volume preceding Trial I amounted to 1,020
minutes/week (41% extensive rowing, 21% nonspecific training, and the rest was
intensive rowing) and preceding Trial II, 880 minutes/week (53% extensive
rowing, 18% intensive rowing, and 11% land training). Performance trials were
evaluated on the first day prior to supplementation and at the end of the
training period (after supplementation) and evaluated how fast the subjects
rowed a 2000 m distance. After an overnight fast, venous blood samples were obtained
before the test, 1 minute after the test, and after a 24-hour recovery period.
Additionally, capillary blood obtained from finger pricks was used to determine
lactic acid. The inflammatory cytokines interleukin-6 (IL-6) and tumor necrosis
factor-alpha (TNF-α) were measured from the serum. Iron concentrations, hepcidin
(master iron regulator), total iron-binding capacity (TIBC), unsaturated
iron-binding capacity (UIBC), myoglobin, ferritin, uric acid, and TAC were also
assessed from the blood samples.
Neither group
differed significantly in age, years of training, body measurements, or the
mean power output and run-time for the 2000-m test performed at the beginning
of the study. Furthermore, no differences were found between the groups for
lactic acid levels before and after supplementation. The post-exercise IL-6
levels were significantly higher than the pre-exercise levels before and after
supplementation for both groups (P<0.05). Pre-exercise TNF-α levels were significantly lower after
supplementation with black chokeberry juice (P<0.05). During the recovery
period and after supplementation, TNF-α was also significantly lower for the group
that consumed black chokeberry juice (P<0.05).
Post-exercise
TAC was significantly lower than pre-exercise TAC for both groups before and
after supplementation (P<0.05). During the recovery period, TAC was
significantly higher in the group that consumed black chokeberry juice compared
to the placebo group (P<0.05). Both groups had significantly higher uric
acid values during the recovery period compared to post-exercise values before
and after supplementation (P<0.05). Post-exercise hepcidin levels were
significantly higher after supplementation for both groups compared to
pre-exercise values (P<0.05). Moreover, the subjects that consumed black chokeberry
juice had significantly higher iron concentrations during the recovery period
(P<0.05). Myoglobin was significantly higher post-exercise for both groups
before supplementation (P<0.05) and no effect was found after
supplementation for either group. No significant effects were observed for
ferritin, TIBC, or UIBC.
The
authors conclude black chokeberry juice (50 mL 3
times a day for 8 weeks) may prevent inflammation, oxidative stress, and iron
depletion after intensive physical exercise, therefore justifying chokeberry
juice supplementation for athletes undergoing rigorous training. These effects are
partially attributed to anthocyanins, a subcategory of flavonoids found in black
chokeberry juice. As physical exercise can cause red blood cells to become more
vulnerable to hemolysis (rupturing of the cell), the authors suggest future
studies include data on the occurrence rate of hemolysis. Larger trials with
male and female subjects are warranted to better understand how black chokeberry
juice consumption impacts inflammatory cytokines, antioxidant status, and iron
metabolism biomarkers after intense exercise.
—Laura M.
Bystrom, PhD
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