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- Aloe Vera (Aloe vera, Xanthorrhoeaceae)
- Propolis
- Dental Cavities
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Date:
10-30-2015 | HC# 051522-531
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Re: Disinfection of Dental Cavities with Aloe Vera or Propolis Extract Significantly Reduces the Number of Bacteria within the Cavities
Prabhakar
AR, Karuna YM, Yavagal C, Deepak BM. Cavity
disinfection in minimally invasive dentistry - comparative evaluation of Aloe vera and propolis: a randomized clinical
trial. Contemp Clin Dent.
March 2015;6(Suppl 1):S24-S31.
Atraumatic
restorative treatment (ART) in dentistry is becoming an increasingly popular
treatment regime in developing countries, for children and for dental patients
with extreme fear of the dentist. ART uses the least invasive techniques for
treatment, including hand tools to remove demineralized portions of the tooth.
If the demineralized material is not completely removed or the cavity is not
properly disinfected, formation of a secondary cavity can occur. Chlorhexidine
is commonly used to disinfect dental caries, but there is evidence that
chlorhexidine can weaken tooth structure. Several natural disinfectants have
been proposed for ART and include aloe vera (Aloe vera, Xanthorrhoeaceae) and propolis, a resinous compound
collected by honeybees (Apis mellifera).
Both have been found to have antimicrobial properties. The goal of this open,
controlled, randomized, split-mouth study was to measure the antimicrobial
properties of aloe vera and propolis in adolescent patients.
Ten
patients between the ages of 5 and 12 years old were recruited from the
Department of Pedodontics and Preventive Dentistry, Bapuji Dental College and
Hospital in Davangere, Karnataka, India. Patients were included if they had
dental caries in a minimum of 3 teeth. Patients were excluded if there was
pulpal involvement, pain or swelling, 1 or more teeth had abscesses, or the
patient had a systemic illness. Patients received ART on each cavity and then
cavities were treated with distilled water, aloe vera extract, or propolis
extract for 60 seconds. The aloe vera extract was prepared from the mucilage produced
in the leaves. The mucilage was dried, extracted with ethanol, filtered, and then
dried to produce the extract. Propolis was extracted with ethanol, filtered,
and dried to produce a sticky, brown substance. Three samples, which included a
sample of the demineralized tooth (baseline), a sample after ART, and a sample
after disinfection, were extracted from each cavity and measured for the total
count of bacteria-forming colonies. Data were analyzed with repeated measures
analysis of variance.
There
was a significant reduction in bacteria-forming colonies after removal of
demineralized tooth material and after disinfection in all treatments when
compared to baseline (P<0.001). The bacterial count decreased from 128.20 ±
47.35 at baseline to 47.20 ± 26.61 after disinfection in the distilled water
treatment, from 142.50 ± 82.23 to 3.90 ± 5.60 in the aloe vera treatment, and from
131.90 ± 51.73 to 4.50 ± 5.23 in the propolis treatment. The final bacterial
counts were significantly lower in the cavities treated with aloe vera or
propolis than in the cavities treated with distilled water (P<0.01).
Treatment
of cavities with ART significantly reduced the number of bacteria within the
cavities, but large numbers of bacteria were still found within the cavities.
Additional treatment with aloe vera extract or propolis extract significantly
reduced the bacterial count within the cavities when compared to distilled
water. Aloe vera and propolis were nearly equivalent in their ability to reduce
bacterial infection within the cavities. Both aloe vera and propolis have been
found to have antimicrobial characteristics. Aloe vera contains a wide range of
anthraquinones that have been shown to be both antibacterial and antiviral.
Propolis contains flavonoids, cinnamic acid, and other compounds that have been
shown to inhibit bacterial growth. This study was limited by the small sample
size.
–Cheryl
McCutchan, PhD
Peer Reviewer
Comments:
The
review does not address the supposition that the aloe vera extract is not
likely to contain constituents found only in the latex.
Note
the procedure used to prepare the extract:
"The leaves of the plant were washed
with distilled water, cut opened, and fresh pulp was collected. The gel was
dried in an oven at 800°C for 48 h and then powdered. An ethanolic extract was
obtained by dissolving 20 g of the powder in 200 ml of ethanol. The contents
were then filtered using Whatman® filter paper no. 1, and the
filtrate was evaporated for dryness."
The paragraph on the mechanism of action
states that "aloin and aloe emodin are the major anthrquinones [sic] in aloe plants … ." and
implies that these constituents are in the extract. However, they are contained
in the discarded rind and are not found in the pulp/gel. So, either other
constituents are responsible for the activity or the pulp/gel was contaminated
with latex during processing. Since no chemical analysis is presented, the
actual case cannot be determined. At least an actual extract was made, an
extract of the inner leaf pulp/gel that should be free of those constituents.
Although the herbal preparation is described, which
should not be unusual but often is, their supposition of active materials is
theoretically incorrect. Authors and reviewers need to address the requirements
for describing botanical materials used in clinical studies, such as the herbal Consolidated Standards
of Reporting Trials (CONSORT) (http://www.ncbi.nlm.nih.gov/pubmed/21208777) or the National Center for Complementary and
Integrative Health (NCCIH) policy on natural product integrity (https://nccih.nih.gov/research/policies/naturalproduct.htm).
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