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- Essential Oils
- Authenticity
- Adulteration
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Date:
01-15-2016 | HC# 071565-536
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Re: Overview of Essential Oil Adulteration
Do TKT, Hadji-Minaglou F, Antoniotti S, Fernandez X.
Authenticity of essential oils. Trends Analyt Chem. March 2015;66:146-157.
Essential oils refer to plant extracts
usually manufactured by various distillation processes or mechanical
expression, and contain aromatic and often volatile compounds. The essential
oils are critical components and ingredients of the flavor, fragrance,
cosmetic, aromatherapy, and medicinal plant industries. Essential oils have a
high commercial value, and certain oils are produced on a small scale (e.g.,
iris [Iris spp., Iridaceae] or damask rose [Rosa × damascena, Rosaceae] oil) and can sell
for over US $10,000/kg due to the scarcity of the raw material. Authentication
of these oils, consisting of a multitude of compounds, is crucial for various
economic and public health reasons. This review describes various techniques
for authenticating these products, which is particularly challenging because of
the variability in essential oils from a given species due to differences in
habitat, harvesting and processing methods, and other sources of heterogeneity.
Many regulatory bodies throughout the world have
created monographs designed to contain specific authentication criteria for
individual essential oils. Adulteration may compromise the value of essential
oils and has come from multiple causes, such as the inclusion of substitute
constituents that are cheaper, the admixture of inexpensive essential oils from
other natural sources, or the addition of vegetable oil or solvents like
propylene glycol, triacetin,
triethyl citrate, or benzyl alcohol. Since the amount of various compounds in essential oils may greatly
affect the economic value of the oil, there is a monetary incentive to spike
certain essential oils with synthetic isolates. For example, the addition of
the compound citral to lemon (Citrus × limon, Rutaceae) essential oil may elevate the quality of the
final product. Mixing synthetic α-bisabolol into a chamomile (Matricaria recutita syn. Chamomilla recutita, Asteraceae)
essential oil to enhance its bioactivity may make this oil more commercially
viable. Essential oils may also be adulterated for olfactory reasons. For
example, lavender (Lavandula angustifolia,
Lamiaceae) essential oil may be spiked with oil from a cheaper species. In
another example, cinnamon (Cinnamomum
verum syn. C. zeylanicum,
Lauraceae) bark essential oil has been adulterated with oil from leaves of the
plant to reduce the amount of the potentially allergenic compound
cinnamaldehyde.
There are many techniques available for the
detection of adulteration. Strategies include investigating the entire chemical
composition (fingerprint) of essential oils or the detection and quantification
of individual marker compounds. One assessment of adulteration comes from
organoleptic experts, specially trained to detect variation in essential oil
products. This method may take time for training and testing. Physical
properties of the essential oils may also be assessed, such as refraction
properties, density, optical rotation, and freezing or boiling points. Organoleptic
assessments, the evaluation of physical properties, and titrations are simple
and fast, but are usually unsuitable to detect more subtle cases of
adulteration.
Chemical separation techniques, such as gas
and high-performance liquid chromatography (GC and HPLC) or high-performance thin-layer
chromatography (HPTLC), may be used to detect the presence and amounts of
individual compounds or to compare chemical fingerprints. GC is especially
useful for essential oil investigation, and chiral GC can further identify
compounds based on their arrangement in space. Other useful techniques include
isotope-ratio mass spectrometry (IRMS), which allows the determination of chemical
compositions of essential oils based on carbon uptake, and nuclear magnetic
resonance (NMR) spectroscopy, which has proven very powerful in confirming the
identity of essential oils.
Chromatographic techniques, which are methods
to separate out compounds, may be paired with mass spectrometry (MS), infrared
(IR) spectroscopy, or Raman spectroscopy to improve detection and provide
additional information about the compounds in the essential oil. Vibrational
spectroscopy uses molecular vibrations to identify covalent bonds, and
multidimensional chromatography couples various chromatography to identify
compounds. Also, differential scanning calorimetry, based on the flow of heat,
and thermal diffusivity are beginning to be used.
Newer data analysis options include complex statistical
evaluations of the chemical analysis (chemometrics) to establish the similarity
between essential oils and to identify adulteration.
In summary, the economically motivated adulteration
of essential oils is an ongoing problem. It is important that the admixture of,
or substitution with, cheaper ingredients is accurately detected, and there are
many analytical techniques available for doing so.
—Amy C. Keller, PhD
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