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- English Hawthorn (Crataegus laevigata syn. C. oxyacantha, Rosaceae)
- Angina Pectoris
- Cardiovascular Disease
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Date:
03-15-2016 | HC# 021661-540
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Re: Hawthorn Extract Reduces Inflammatory Cell Adhesion Molecules in Patients with Stable Angina
Jalaly
L, Sharifi G, Faramarzi M, et al. Comparison of the effects of Crataegus oxyacantha extract, aerobic
exercise and their combination on the serum levels of ICAM-1 and E-selectin in
patients with stable angina pectoris. Daru.
December 19, 2015;23:54. doi:
10.1186/s40199-015-0137-2.
Stable angina (angina pectoris) refers to heart
pain caused by a lack of oxygen availability, and is preceded by inflammation
and atherosclerosis. Cell adhesion molecules (CAMs) facilitate the inflammation
process with white blood cell recruitment, and can negatively affect cellular
and tissue function. Previous studies have shown English hawthorn (Crataegus laevigata syn. C. oxyacantha, Rosaceae), and other
species of Crataegus, to be useful in
treating symptoms of heart failure.1 This randomized, double-blind,
placebo-controlled trial investigated the impact of hawthorn consumption,
combined with aerobic exercise (also shown to reduce CAMs), on intercellular
adhesion molecule-1 (ICAM-1) and E-selectin. These CAMs have been shown to be
important in the pathology of cardiovascular disease.
English hawthorn leaf and flower extract (EPO
Istituto Farmochimico Fitoterapico S.r.l.; Milan, Italy) was used for this
study. The product Cratagol (provided by Goldaru; Isfahan, Iran), consisting of
240 mg of extract per tablet standardized to 4-6 mg of vitexin-2-ramnozide, was
used. These tablets also contained microcrystalline cellulose, corn (Zea mays, Poaceae) starch, talc, and
magnesium stearate. Placebo tablets (Goldaru) consisted of lactose with identical
coating. Two tablets were administered per day with water, prior to meals, for
12 weeks.
Patients diagnosed with stable angina were
recruited from the Imam Ali Clinic in Shahrekord, Iran. Patients were included
if they had received angina testing between 1-12 months prior to the study,
< 50% atherosclerosis, treatment within 3 months prior to the study, and if
they were 45-65 years old. Those with severe heart failure, "lack of
accessibility," or who were taking medications, including digoxin,
cisapride, anticoagulants, or antiarrhythmics, were not included. In total, 80
patients were randomly assigned to 1 of 4 groups with 20 patients in each (11
men and 9 women) as follows: (1) exercise with placebo, (2) hawthorn extract
alone, (3) exercise and hawthorn extract together, and (4) control (no
exercise, hawthorn extract, or placebo). At baseline, there were no differences
in age, sex, weight, or body mass index among groups. Exercise, nutrition,
smoking and drinking habits, and angina symptoms were monitored.
Throughout the study, all patients received 50
mg/day of Methoral, 80 mg/day of aspirin, and nitroglycerine as needed for pain.
Exercise consisted of a treadmill protocol of 40-60% of heart rate reserve and an
11-13 rating of perceived exertion using the Borg scale (this scale ranges from
6 to 20, where 20 indicates maximal exertion). The exercise protocol was done
for 20-30 minutes twice per week for 12 weeks. If patients had chest pain,
asthma, dizziness, fatigue, or decreased systolic blood pressure by > 10
mmHg, exercise was halted. Fasting blood was collected at baseline (24 hours
prior to the study) and at the end of the study. Enzyme-linked immunosorbent
assay (ELISA) kits were used to measure serum concentrations of ICAM-1 and
E-selectin.
None of the patients dropped out. At baseline,
ICAM-1 and E-selectin were not different among groups. At the end of the study,
ICAM-1 significantly decreased compared to baseline concentrations in the
exercise group (65.5 ± 39.7 ng/ml vs. 20.8 ± 2.7 ng/ml, P=0.001), hawthorn extract
group (61.3 ± 38.1 ng/ml vs. 23.1 ± 3.7 ng/ml, P=0.001), and the exercise and hawthorn
extract combined group (90 ± 53.5 ng/ml vs. 21.9 ± 3.9 ng/ml, P=0.001). No
significant differences were noted in the control group. Also, as compared to
baseline, E-selectin concentrations were significantly decreased in the
exercise group (3.2 ± 1.5 ng/ml vs. 1.8 ± 1 ng/ml, P=0.001), hawthorn extract
group (3.4 ± 1.9 ng/ml vs. 2.2 ± 1.3 ng/ml, P=0.003), and the exercise and hawthorn
extract combined group (3.5 ± 1.3 ng/ml vs. 1.8 ± 0.7 ng/ml, P=0.001). No
differences were seen in the control group.
The ICAM-1 serum concentrations of those in the
exercise and hawthorn extract combined group were significantly lower than those
taking hawthorn extract alone (P=0.021) and the control group (P<0.05), but
not the exercise-only group. Also, E-selectin concentrations were lower in the
combination group than in the hawthorn extract-only group and the control group
(P=0.021 and P<0.05, respectively), but not the exercise-only group. No
adverse side effects were noted.
In summary, this study shows that both English
hawthorn extract and exercise were effective in attenuating the concentration
of ICAM-1 and E-selectin. This may be due to the alleviation of downstream
inflammation markers or general bioactivity associated with the antioxidant
potential of hawthorn. It is curious that the control group in this study did
not receive the placebo; this somewhat confounds the results. However, English
hawthorn extract may be an effective adjuvant treatment, with or without exercise,
for those suffering from stable angina.
—Amy C.
Keller, PhD
Reference
1Veveris M, Koch E, Chatterjee SS. Crataegus
special extract WS® 1442 improves cardiac function and reduces
infarct size in a rat model of prolonged coronary ischemia and reperfusion. Life Sci. February 27, 2004;74(15):1945-1955.
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